Studying Impact of Vitamins Application on Growth of Wheat Plantlets Cultured In vitro

The present study aimed to investigate physiological and biochemical responses of wheat plantlets to application of concentrations of vitamins. In general, the data point out that addition of low concentrations of vitamins to MS media greatly improved most vegetative growth criteria concerned of wheat plantlets culture in vitro via plant tissue culture technique. In the current study root length (7.53 cm), shoots fresh weight (0.480 g), total chlorophyll content (380 μg g-1 FW), soluble sugars (6.88 mg glucose g-1 DW), and soluble proteins (4.9 mg protein g-1 FW) enhancement in in vitro of wheat plantlets with application low concentrations of vitamins on the contrast, the highest concentration of the same vitamins, decreased their physiological and biochemical characteristics compared to control.