Determination of amino-acidic positions important for Ocimum basilicum geraniol synthase activity

Fischer, Marc J. C. and Meyer, Sophie and Claudel, Patricia and Steyer, Damien and Bergdoll, Marc and Hugueney, Philippe (2013) Determination of amino-acidic positions important for Ocimum basilicum geraniol synthase activity. Advances in Bioscience and Biotechnology, 04 (02). pp. 242-249. ISSN 2156-8456

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Abstract

Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum. The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation.

Item Type: Article
Subjects: AP Academic Press > Biological Science
Depositing User: Unnamed user with email support@apacademicpress.com
Date Deposited: 14 Mar 2023 10:56
Last Modified: 07 Sep 2024 10:05
URI: http://info.openarchivespress.com/id/eprint/780

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