Hepatoprotective Effect of Annona Squamosa Linn. (AS) Leaves on Isoniazid & Rifampin Induced Hepatotoxicity in Rats

The goal of this investigation was to examine if an ethanolic extract of Annona squamosa has any hepatoprotective benefits in rats suffering from isoniazid-rifampin induced hepatotoxicity.

Methodology: The rats were separated in five groups (n=6): group 1, a control; group 2 administered isoniazid (100 mg/kg, I.P.) & rifampin (100 mg/kg, I.P.) in sterile water; group 3 as a control & traditional silymarin, 2.5 mg/kg, B.W., P.O.; groups 4 & 5 are treated & received 200 & 400 mg/kg, B.W., P.O., ethanolic extract of AS. All of the treatment procedures were monitored for a total of 21 days after the rats were slaughtered & Biochemical & histological investigations were performed on the blood & liver respectively.

Results: Rats (Group - 2) treated with Rifampin (RIF) & Isoniazid (INH) elevated their blood Serum Glutamic Oxaloacetic Transferase (SGOT), Serum Glutamate pyruvic transaminase (SGPT) & Alkaline Phosphatase (ALP) levels during a variety of ways. The consumption of ethanolic extracts of Annona squamosa considerably reduced Rifampin & Isoniazid induced elevations in serum diagnostic liver marker enzymes. SGOT, SGPT & ALP are the three involved enzymes. Furthermore, the medical care groups had significantly larger total macromolecule & total albumen levels. The extract result was compared thereto of Silymarin, a standard medication. The histological profile backed up the changes in biochemical markers. An ethanolic extract of Annona squamosa has been discovered to protect rats from oxidative liver injury caused by rifampin & isoniazid.

Conclusion: The ethanolic extract of AS protects rats from isoniazid & rifampin induced oxidative liver injury.