Tagger—A Swiss army knife for multiomics to dissect cell type–specific mechanisms of gene expression in mice

Kaczmarczyk, Lech and Bansal, Vikas and Rajput, Ashish and Rahman, Raza-ur and Krzyżak, Wiesław and Degen, Joachim and Poll, Stefanie and Fuhrmann, Martin and Bonn, Stefan and Jackson, Walker Scot and Gilbert, Wendy V. (2019) Tagger—A Swiss army knife for multiomics to dissect cell type–specific mechanisms of gene expression in mice. PLOS Biology, 17 (8). e3000374. ISSN 1545-7885

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Abstract

A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.

Item Type: Article
Subjects: AP Academic Press > Biological Science
Depositing User: Unnamed user with email support@apacademicpress.com
Date Deposited: 23 Jan 2023 07:51
Last Modified: 17 Jul 2024 09:26
URI: http://info.openarchivespress.com/id/eprint/267

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