Ojowu, John and Ortese, Michael and Etim, Esin (2017) Enzyme Hydrolysis of Hydrogen Peroxide Pretreated Lignocellulosic Content of Rice Husks Prior to Bioethanol Formation. Asian Journal of Biology, 3 (3). pp. 1-6. ISSN 24567124
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Abstract
Aims: This work was aimed at evaluating the production of sugar from the bio-fermentation of rice husk using hydrogen peroxide as a pre-treatment medium.
Methodology: Different samples of rice husks were pretreated with 1%, 1.5%, 2%, and 2.5% (w/v) hydrogen peroxide respectively and allowed to delignify for 2 hours prior to saccharification by cellulose at 37°C and pH of 4.7. The experiment was repeated at varying time intervals spanning 2-24 hours. Amount of reducing sugars produced was determined spectrophotometrically and documented.
Results: High reducing sugar yields at concentrations of 1%, 1.5%, and 2%, H2O2 were observed as 0.81 mg/ml with saccharification of 10.47%, 1.15 mg/ml with saccharification of 14.87%, and 1.42 mg/ml with saccharification of 18.36% respectively. Pretreatment with 2.0% H2O2 was found to achieve highest reducing sugars yield (1.42 mg/ml with saccharification of 18.36%) after pretreatment time of 8 hours as against other concentrations.
Conclusion: The result suggests that degree of saccharification is dependent on concentration of hydrogen peroxide used and time of pre-treatment. The reduction in sugar yield at 2% H2O2 may be as a result of hydrogen peroxide solubilizing lignin which may act as an inhibitor during enzyme hydrolysis.
Item Type: | Article |
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Subjects: | AP Academic Press > Biological Science |
Depositing User: | Unnamed user with email support@apacademicpress.com |
Date Deposited: | 26 May 2023 05:44 |
Last Modified: | 07 Sep 2024 10:06 |
URI: | http://info.openarchivespress.com/id/eprint/1258 |