Enzyme Hydrolysis of Hydrogen Peroxide Pretreated Lignocellulosic Content of Rice Husks Prior to Bioethanol Formation

Ojowu, John and Ortese, Michael and Etim, Esin (2017) Enzyme Hydrolysis of Hydrogen Peroxide Pretreated Lignocellulosic Content of Rice Husks Prior to Bioethanol Formation. Asian Journal of Biology, 3 (3). pp. 1-6. ISSN 24567124

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Abstract

Aims: This work was aimed at evaluating the production of sugar from the bio-fermentation of rice husk using hydrogen peroxide as a pre-treatment medium.

Methodology: Different samples of rice husks were pretreated with 1%, 1.5%, 2%, and 2.5% (w/v) hydrogen peroxide respectively and allowed to delignify for 2 hours prior to saccharification by cellulose at 37°C and pH of 4.7. The experiment was repeated at varying time intervals spanning 2-24 hours. Amount of reducing sugars produced was determined spectrophotometrically and documented.

Results: High reducing sugar yields at concentrations of 1%, 1.5%, and 2%, H2O2 were observed as 0.81 mg/ml with saccharification of 10.47%, 1.15 mg/ml with saccharification of 14.87%, and 1.42 mg/ml with saccharification of 18.36% respectively. Pretreatment with 2.0% H2O2 was found to achieve highest reducing sugars yield (1.42 mg/ml with saccharification of 18.36%) after pretreatment time of 8 hours as against other concentrations.

Conclusion: The result suggests that degree of saccharification is dependent on concentration of hydrogen peroxide used and time of pre-treatment. The reduction in sugar yield at 2% H2O2 may be as a result of hydrogen peroxide solubilizing lignin which may act as an inhibitor during enzyme hydrolysis.

Item Type: Article
Subjects: AP Academic Press > Biological Science
Depositing User: Unnamed user with email support@apacademicpress.com
Date Deposited: 26 May 2023 05:44
Last Modified: 07 Sep 2024 10:06
URI: http://info.openarchivespress.com/id/eprint/1258

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